HPLC法測(cè)定參芪通絡(luò)顆粒中羥基紅花黃色素A的含量

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【中圖分類(lèi)號(hào)】R284.1 【文獻(xiàn)標(biāo)志碼】A 【文章編號(hào)】1007-8517（2025）11-0052-03

DOI： 10.3969/j . issn.1007-8517.2025.11. zgmzmjyyzz202511011

Determination of Hydroxysafflower Yellow A in Shenqi Tongluo Granules by HPLC

LIN Qunfeng'XU Jie2*ZHANG Huijing2 LI Ning2LI Qiang2 1. ， ， ，; 2. ， ，

Abstract：ObjectiveToestablishahighpeformance liquidchromatographymethodforthequantificationof hydroxysafflower Yellow A in Shenqi Tongluo granules. Methods After dissolved with 50% methanol，ultrasonic， filtered，the test sample was loaded onto a YMC - Triart C18 （ 250mm×4.6mm ， 5μm ）column to separate with a mobile phase of methanol -acetonitrile -0.7% （2 phosphoric acid（30：2：68），the detection wavelength was 403nm ，the column temperature was 30 C ，the injection volume was 10 （20 μL ， the flow rate was 1.0mL/min . Results The calibration curve of hydroxysafflower yellow A showed a good linearity（ R2=1 ） in the range of32.55-651 ng.The recoverieswere in the range of100.17%-102.64 % ， the relative stard deviations（RSD） was 0.87% .Conclusion The method issimple，accurate，reproduciblestable，can beused forthedeterminationof hydroxysafflower yellow A in Shenqi Tongluo granules.

KeyWords：Shenqi Tongluo Granules；Hydroxysafflower Yellow A；Content Determination；HPLC

參芪通絡(luò)顆粒處方來(lái)源于壽光市中醫(yī)醫(yī)院臨床經(jīng)驗(yàn)方。（剩余4235字）