實(shí)時熒光PCR法檢測芝麻醬中的芝麻成分

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摘 要：利用實(shí)時熒光PCR法檢測芝麻醬中的芝麻源性成分，以鑒定預(yù)包裝產(chǎn)品的真?zhèn)巍＿x取3份市售芝麻醬產(chǎn)品，其中1份標(biāo)示不含芝麻成分，利用熒光定量PCR法進(jìn)行檢測。結(jié)果顯示該法檢測結(jié)果與產(chǎn)品標(biāo)簽相符，建立的檢測方法可行。

關(guān)鍵詞：芝麻醬；芝麻成分；實(shí)時熒光PCR法

Real-Time Fluorescence PCR Method for Detection of Sesame Components in Sesame Paste

LI Juan1， CHEN Yinyin2， MEI Yan1， CHEN Xuehua1， WANG Da1， HUANG Jiawen1

（1.Guangdong Food and Drug Vocational-Technical School， Guangzhou 510000， China; 2.Guangdong Provincial Institute of Food Inspection， Guangzhou 510000， China）

Abstract： A real-time fluorescence PCR method was used to detect sesame-derived ingredients in sesame paste to identify the authenticity of prepackaged products. Three commercially available sesame paste products， one of which was labeled as not containing sesame ingredients， were selected and tested using a fluorescence quantitative PCR method. The results showed that the assay results were consistent with the product labels and the established assay was feasible.

Keywords： sesame paste; sesame components; real-time fluorescence PCR

芝麻又稱胡麻，既是油料作物，又可直接食用。（剩余3605字）