藏公雞MC1R基因多態(tài)性與羽色表型的關(guān)系
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關(guān)鍵詞：藏公雞；黑素皮質(zhì)素受體1（MC1R）基因；基因多態(tài)性；羽色表型中圖分類號(hào)：S831.2 文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：0439-8114（2025）05-0173-05DOI：10.14088/j.cnki.issn0439-8114.2025.05.027開放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）：
Relationship between MCiR gene polymorphism and plumage color phenotype in Tibetan roosters
WANG Shi-xin 1，2 ，GAO Ming-xin2，Danzeng Sangzhu2，Ba-sang，CHEN Xi3，WANG Run-jin2， ZHANGHao1，WANGDe-cai，PIJin-song1，HUANG Tao1
（1.InstituteofAialHusbandryndVetearyHubeiAcadeyofcuraliencs，Wuhan4o，ina；.aanbetaick Industryesechiute6ta;aalbddeinarin China；4.Agricultural Industrialization Service Center of Jiangxia District，Wuhan 43O2Oo，China）
Abstract：AdultTibetanrosters withdistinctplumagecolorphenotypes（Tibetanblack，Tibetanvariegated，andTibetangray）were selectedtoscreengeneticvariationsitesinthecodingregionof themelanocortin1receptor（MC1R）geneandanalyzegenotype-phenotypecorrlatios.Teresultsshowedthat10SPswereidentifedinthecodingregionoftheMClRgeneiTibetanroosters，cluding2 synonymous mutations at69bp（C/T）and 636bp（A/G），and8non-synonymous mutationsat178bp（A/G），212bp（C/T）， 213bp（A/G），274bp（A/G），376bp（A/G），398 bp（C/T），427bp（A/G），and 637bp（C/T）.Genotype distributionrevealed hat all Tibetan black roosters exhibited the AA genotype （ 100.00% ）， 60.00% of Tibetan gray roosters carried the AA genotype，while Tibetan variegated roosters predominantly had the AG genotype （ 80.00% ），suggesting that the A allele might be associated with the formationof the black plumage phenotype.Thirten haplotypes were constructed based on the10 SNPs，withhaplotypes Hap_2，Hap 10，andHap_13exclusivelyobservedinTibetanvariegatedroostersChi-squareindependence tests indicatedthatgenotypsat69bp （C/T）and 274 bp（A/G）were significantly associated with plumage color phenotypes （ P<0.001 ），suggesting these two loci might serve as key genetic markers regulating plumage color variation in Tibetan roosters.
Key Words：Tibetan rooster;melanocortin l receptor（MC1R）gene；gene polymorphism； plumage color phenotype
羽色是禽類重要的表型性狀，具有重要的經(jīng)濟(jì)價(jià)值。（剩余7100字）