干燥方式對煙花有機酸含量的影響及判別分析
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（1.中國農(nóng)業(yè)科學院煙草研究所，青島266101；2.中國農(nóng)業(yè)科學院研究生院，北京100081；3.山東臨沂煙草有限公司，山東臨沂276000；4.山東中煙工業(yè)有限責任公司技術中心，濟南250013)
中圖分類號： 文獻標識碼：A
文章編號：1007-5119（2025）02-0069-06
Effects of Drying Methods on Organic Acid Contents of Tobacco Flowers and Discriminant Analysis
XU Minglei1,2, HOU Xiaodong1, ZHANG Zhongfeng1, TIAN Lei3*,WEI Yulei4,HAN Xiao1, TAN Xiaolei3,DU Yongmei1* (1.Tobacco ResearchInstituteofCAAs,Qingdao266101,China;2.Graduate SchoolofChineseAcademyofAgriculturalSiences, Beijing100081,China;3.ShandongLinyiTobaccoCo.,Ltd.,Linyi2760oo,Shandong,China; 4.TechnologyCenterofChina Tobacco Shandong Industry Co.,Ltd., Jinan 25oo13,China)
Abstract: Toexplore the utilizationoftobaccoflower in tobacco flavoringapplications and establish disriminant modelfordrying methods basedon keyorganic acids,GC/MS was employed toanalyze the diferences inorganic acid content acrossseven drying methods.Partialleastsuaes-dimantaalysis(-)asedtoidntifyeydirentialoganiccis,flodbyisher discriminantanalysis toconstructadiscriminantmodel foridentifyingdrying methods.Resultsshowedas thefollows.(l)Volatile acidsintobaccoflowerstreatedwithdiferentdryingmethodswereidentifiedasbenzoicacid，phenylaceticacidand3- methylpentanoic acid (with characteristic aroma), with total content ranging from 116 to 1 8 1 μ g / g . The total content of non-volatile acids ranged from 59.7 to 7 7 . 0 m g / g ，with the sum of oxalic acid, malic acid and citric acid content accounting for approximately 9 2 . 5 % to 9 7 . 8 % of the total. The total content of higher fatty acids ranged from 22.4 to 6 5 . 2 m g / g ,with unsaturated fatty acids (oleic acid,linoleic acid,and linolenic acid) accounting for about 5 8 . 7 % to 7 6 . 6 % of the total. (2) Drying methods significantly affected organic acidcontents: airdrying achieved thehighest volatile acid content,folowed byprogrammed heating drying andsun drying, whereas freeze drying yielded the lowest.Non-volatileacidsreached peak levels during freeze drying,whereastheir minimum content were observed in constant temperature drying. The total content of higher fatty acids was the highest in freeze drying andthelowest inairdrying.(3)PLS-DAclasifiedthesevendrying metodsintofivecategories:freezedrying,programmedheating drying,constanttemperaturedrying,sundrying,andairdrying.BasedontheVIPvaluesfromthePLS-DAmodel,sevenkey diferentialoanccids,elytaricid,leiccidiccidliccidiriccid,sticcidndacid wereidentifiedascharacteristicmarkersSubsequetlyaFisherdisrimiantmodelwasestablishdforthfivecategorsoftobac flower drying methods, with classification accuracies of 9 6 . 7 % in the training set and 1 0 0 % in the test set. In summary, the organic acid profiles of tobacco flowers subjected to air drying,sun drying,or programmed heating drying (up to ）exhibitgreater suitabilityfortobacoflavoringapplications.Organicacidscanserveascharacteristicchemicalcomponentsforeffectively discriminating tobacco flower resources obtained from different drying methods.
Keywords: tobacco flowers; drying method; organic acid; PLS-DA; Fisher discriminant analysis
烤煙是我國重要的經(jīng)濟作物。（剩余14840字）