叢枝菌根真菌種類和接種方式對烤煙幼苗生長的影響
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中圖分類號：S572.01文獻標識碼：A
文章編號：1007-5119（2025）02-0009-09
Effect of Arbuscular Mycorrhizal Fungi Species and Inoculation Methods on Growth of Flue-Cured Tobacco Seedlings
ZHANG Yuyin1， YANG Jie1， GE Zixuan1， LI Yingying1， GUAN Kunhao1， ZHANG Tongjin2， ZHANG Yujie1， DAI Xiaoyan1* （1.CollegeofTobacco Science，Henan Agricultural University/KeyLaboratoryforTobacco CultivationinTobacondustry， Zhengzhou 450，China; 2.Bijie Branch of Guizhou Provincial Tobacco Company，Bijie 551700，Guizhou， China）
Abstract：Thisstudyaimstoclarifytheroleofarbuscular mycorhizalfungi（AMF）inthegrowthandphysiologicalcharacteristics offlue-cured tobaccoseedlings.Apotexperiment wasonductedtodetecttheimpacts of treediferentinoculationmethods andfive AMFstrains（Acaulosporascrobiculata，As；Rhizophagusintraradices，Ri；Fumeliformismosseae，F(xiàn)m；Claroideoglomus etunicatum，Ce; Glomusvesiforme，Gvonthegowth prameters，potosythticpeformance，cabonandnitrogenmetabolis，and mineralnutrientuptakeoftheflue-curedtobacoVarietyK326seedlings.ResultsshowedthatthefiveAMFstrainsestablished symbiotic relationships withthe tobaccoroots under thethreeinoculation methods.Compared tothenon-inoculatedcontrol，AMF inoculationsignificantlyicreasedtheootprojectionarea，totalrotlength，oottipumber，drymaterccumulationndnet photosyntheticrateoftobaccoplants.In particular，underthe inoculation methodthree（where theinoculumwas placedapproximately away from the tobacco roots），the tobaco seedlings inoculated with Acaulospora scrobiculata （As）exhibited significantly highertotalootlength，otprojetionarea，dryateraccumulation，andetphotosytheticatethantoseinocuatedwithother AMFstrains.Furthermore，underthemethodthree，thesedlingsinoculatedwithAsdisplayedsignificantlyhigherlevelofroot diameter，drymateracumulation，netphotosyntheticrate，sucrose phosphatesynthaseactivityandnitrogencontent inthe underground parts，compared tothose inoculatedunderthe methodtwo（wheretheinoculum was indirectcontact withtheroots）. These findingssuggesthat AMFinoculation can efectivelypromote theotandleaf developmentofflue-cured tobaccoseedlings， andenhanceitsnutrientuptake.TheAsstrain，wheninoculatedunderthemethodthree，forms themost beneficialsymbiotic relationship， yielding superior results.
Keywors：arbuscularmycorzalfungi;fluecuredtobaco;ioculationmethod;growth;physiologicalcharacteristics;ieral nutrients
烤煙壯苗培育是優(yōu)質(zhì)煙葉生產(chǎn)的基礎，直接影響煙株大田生長發(fā)育及煙葉產(chǎn)量和品質(zhì)[1]。（剩余17101字）