長沙煙區(qū)煙草赤星病病害調(diào)查與病原菌鑒定
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中圖分類號：S435.72 文獻(xiàn)標(biāo)識碼：A文章編號：1007-5119（2025）02-0045-07
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(1.湖南農(nóng)業(yè)大學(xué)植物保護(hù)學(xué)院，長沙，410128；2.湖南省煙草公司長沙市公司，長沙，410023；3.湖南省長沙市農(nóng)業(yè)農(nóng)村局，長沙，41000；4.湖南省微生物研究院應(yīng)用真菌室，長沙410009）
Investigation of Tobacco Brown Spot Disease and Pathogen Identification in Changsha Tobacco-Growing Region
XIE Lin1, CAI Hailin2, LI Jianyong2, ZHAI Zhengguang2, ZOU Shuo3,HE Haoxin1,FU Jing1, HUANG Binbin4,LIU Qingshu4,CHEN （20
(1.College ofPlantProtection,HunanAgriculturalUniversityChangsha4028,China;2.ChangshaBranchofHunanobacco
Company,Changsha,410o3,China;3.ChangshaMunicipalBureauofAgricultureandRuralAairsofHunanProvince,Cangsha 410000,China; 4.Aplied MycologyLaboratory,Hunan Provincial Instituteof Microbiology,Changsha 4109,China)
Abstract: Brown spot disease is one of the primary factors afecting safety of leaf tobaccoquality in Changsha tobacco-growing region.Toinvestigate theocurenceandidentifythepathogenicspeciescausing tobacobrownspotdisease,acomprehensivesurvey wasconductedonincidencerateand diseaseindexofbrownspot diseaseacrossthe tobacco production towns in Changsha.Results showed that the incidence rate and disease index ranged 0 . 5 6 % - 1 6 . 6 7 % and 0.06-5.43 respectively. Through combined morphological andmolecularbiologicalidentificationofsolatedpathogens,thestudyrevealedthatthecausativeagentsereAlternariaalteata and A. tenuissima, with the detection rate of A .tenuissima up to 7 5 . 0 0 % . Further in this study, rDNA-ITS gene was confirmed to be excesive conservationforspecies-leveldiferentiation withintheAlternariagenus,whereastheplasma membraneATPasegene displayedconcordance withmorphologicalidentificationresults,provingsuitableforinterspeciesdiscriminationInconclusion,the pathogensoftobaccobrownspotdiseaseinChangshatobacco-growingregionexhibithighdiversity,withAlternariaalernatabeing predominant.
Keywords: Changshatobaco-growingregion; tobaccobrowspotdisease;pathogenidentification;A.alternata,A.tessima
煙草赤星病(tobaccobrownspot)主要發(fā)生在煙葉打頂后的成熟階段，具有潛伏期短、暴發(fā)快等特點，適宜條件下短時間內(nèi)可造成大面積流行[1]。（剩余14261字）