高效液相色譜法測(cè)定面包中丙酸及丙酸鹽的含量
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Determination of Propionic Acid and Propionates in Bread by High-Performance Liquid Chromatography
ZHANG Yu， LIU Ying* （Tianjin Institute of Food Safety Inspection Technology， Tianjin 30o308， China）
Abstract： In view of the serious matrix interference， poor repeatability， and difficulty in qualitative and quantitative determination of propionic acidand propionate in existing food detection methods，this study optimized the HPLC detection method for propionic acid and propionate in bread.The sample was mixed with water and 1mol?L-1 phosphoric acid solution and then ultrasonically extracted. The pH value was adjusted to about 3. The supernatant was filtered through a microporous filter membrane and separated by a C18 chromatographic column. Phosphoric acid aqueous solution and methanol were used as the mobile phase for gradient elution and the determination was performed bya diode array detector.The results showed that the detection limitof this method was （20 0.02g?kg-1 ，the quantification limit was 0.08g?kg-1 ，and the recovery rate was 91.5%～105.0% when the content of propionic acid and propionate was 0.08～2.50g?kg-1 ，and the relative standard deviation was 2.29%～4.79% This method is simple and fast to operate，can efectively separate the target peak and impurity peak inthe bread matrix， and the determination results are accurate and stable.
Keywords： high-performance liquid chromatography; propionic acid; propionates; bread
丙酸是一種無(wú)色透明且具有一定腐蝕性的液體，帶有刺激性氣味，被廣泛應(yīng)用于飼料防腐和食品防腐領(lǐng)域[1]。（剩余4275字）