基于離子液體與微波輔助的川續(xù)斷提取液抑菌作用及機(jī)制研究

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中圖分類號(hào)：TS210.9 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1671-8755（2025）02-0025-08

Study on the Antibacterial Effect and Mechanism of Dipsacus Asper Extract Based on Ionic Liquid and Microwave-assisted Method

ZOU Jing1 ，KANG Jianqing'， ZHOU Mengjiao2， ZHANG Yu' ，KANG Ming'，LIANG Xiaofeng1，2

（1. School of Materials and Chemistry，Southwest University of Science and Technology，Mianyang 621010， Sichuan， China; 2. Mianyang Key Laboratory of Development and Utilization of Traditional Chinese Medicine Resources， Sichuan College of Traditional Chinese Medicine，Mianyang 621olo， Sichuan，China）

Abstract：Using Dipsacus as the raw material，the extract was obtained through ionic liquid and microwave-assisted extraction technology.Antibacterial experiments and active component analysis of the extract were conducted，and the antibacterial mechanism was discussed.The results show that the Dipsacus extract exhibits antibacterial effects against Escherichia coli（ E . coli），Staphylococcus aureus （S. aureus），and Salmonella paratyphi β （ S .paratyphi β ），with its inhibitory activity being concentration-dependent. The inhibition zone diameters for E . coli，S.aureus，and S . paratyphi β are 27 mm，39 mm，and 19mm ，respectively. The minimum inhibitory concentration （MIC）and minimum bactericidal concentration（MBC） against S. aureus are determined to be 3.1250mg/mL and 6.250 0mg/mL ， respectively. For E ： coli，the MIC and MBC values are 3.1250mg/mL and 6. 250 0mg/mL ， respectively. For S ：paratyphi β ，MICis 6.250 0mg/mL and MBC is 12.500 0mg/mL . Chromatographic analysis reveals the presence of antibacterial components in Dipsacus extract，including chlorogenic acid，caffeic acid，and asperosaponin VI. Morphological observations suggest that the antibacterial mechanism may involve disruption of bacterial cell wals and membranes.These findings provide valuable references for the development of antimicrobial agents from Dipsacus.

Keywords： Dipsacus asper extract；Antibacterial constituent； Antibacterial activity； Antibacterialmechanism

植物中的皂苷類、酚酸類成分（綠原酸、咖啡酸等）對(duì)多種革蘭陽性和革蘭陰性細(xì)菌均具有顯著的抑菌作用[1-4]，其抑菌機(jī)制可能與降解細(xì)菌細(xì)胞壁結(jié)構(gòu)、破壞細(xì)胞膜完整性導(dǎo)致胞內(nèi)物質(zhì)外泄并引發(fā)細(xì)菌死亡相關(guān)[5]

川續(xù)斷（DipsacusasperWall.exHenry）是川續(xù)斷科多年生草本植物的根，富含皂苷成分，以“續(xù)折接骨"之功效而聞名[6，是一種重要的中藥材。（剩余10163字）