低溫脅迫下玉米根系轉(zhuǎn)錄組和代謝組分析
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中圖分類號：S513.01 文獻(xiàn)標(biāo)識碼：A 文章編號：1000-4440（2025）06-1063-09
Abstract：Cold stress is one of the primary abiotic stresses limiting the growth of maize seedlings.Inorder to study theeffectoflowtemperaturestressonmaizeroots，thisstudyusedmaizeinbredlineB73astheexperimentalmaterial，and dynamically monitored the growth of the primary root at different time points under control（28 C day/22 C night）and low-temperature treatment （ 15‰ day/ 10qC night）.Additionally，we determined the transcriptome and metabolome of the rottissues from both thecontrol groupand the1-daylow-temperature treatment group.Phenotypic characterizationresults indicatedthatthe growthof the primaryroot was significantly inhibitedunderlow-temperature stress.Metabolomic analysis identified 57diferentiallyaccumulated metabolites，predominantlyenriched in starchand sucrose metabolism pathways.A total of 2 769 differentially expressed geneswere identified bytranscriptomeanalysis，which weremainlyinvolved in starch and sucrose metabolism and phenylalanine metabolism.Further integrationof transcriptomic andmetabolomic analyses revealed that starch and sucrose metabolismwere keypathwaysmediatingroot adaptationtolow-temperature stress.Under low-temperature stress，the content of soluble
sugarssuchasfructoseandglucosesignificantlyincreasedAtotalof26diferentiallyexpressed genes wereidentifiedinthe starchand sucrose metabolicpathways，with12 genesshowing significant upregulationand14 genesshowing significant downregulation.Thisstudyprovidesatheoretical foundationandcandidategenes forfurther investigation intothemolecular mechanisms of maize root adaptation to low-temperature stress and for genetic improvement.
Key words： maize；low temperature stress；transcriptomics；metabolomics
植物的生長和發(fā)育依賴于農(nóng)田環(huán)境，在植物生長過程中常受到干旱、高溫、低溫、洪澇等非生物脅迫的影響[1]。（剩余15595字）