小麥TaLFNR1-7A基因的生物信息學(xué)及表達(dá)分析
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中圖分類號：S512 文獻(xiàn)標(biāo)識碼：A 文章編號： 1000-4440（2025）06-1041-09
Abstract：Feritin-NADP+reductase（FNR）isan important enzyme that playsa crucial role inphotosynthesis and cellularrespiration processs.Thisstudyusedcommon wheatChinese Spring as the materialandcloned the TaLFNR1-7A geneusing RT-PCR technology.Bioinformaticsand gene expresion analysis were performedon it.The resultsshowed that the CDS sequence length of TaLFNR1-7A gene was 1O86bp，encoding 361 aminoacids.The encoded protein was a nontransmembrane stablehydrophilicprotein，withoutasignalpeptide，containing atotalof39phosphorylation sites.It was predicted tobelocalizedinthechloroplast.Phylogeneticanalysisandmultipleaminoacid sequence alignmentrevealed that common wheat（Chinese Spring）and emmer wheat exhibitedcloserphylogenetic proximity basedon the TaLFNR1-7A gene，with a sequence similarity of 97.01% . Analysis of promoter cis-acting elements revealed that the TaLFNRl-7A gene contained17cis-acting elements，including seven light-responsive elements.TheqRT-PCRanalysisrevealed that the expresionof TaLFNR1-7Agene was highestin wheat leaves，folowedbystems and germs，with thelowestlevelobserved in
roots.TheTaLFNR1-7A generespondedtomultipleabioticstresses，including drought（PEG-6OOO treatment），methyl jasmonate（MeJA），sodiumchloride（NaCl），andabscisicacid（ABA）.The findings ofthisstudyprovideasignificantfoundation forfurther exploringthe functionalmechanisms of the TaLFNR1-7A gene in wheat growth anddevelopment.
Keywords：wheat；TaLFNRl-7A gene；bioinformatics；gene cloning；expression analysis
小麥（TriticumaestivumL.）作為全球主要糧食作物之一，在世界范圍內(nèi)具有不可或缺的地位。（剩余11607字）