菊花腦CnNAC83基因克隆及其表達(dá)分析

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【Abstract】Using stemand leaf tissues of Chrysanthemum nankingense，we clone the NAC transcription factor gene CnNAC83and perform bioinformaticsanalysis along with expressonprofiling underPEG-6Ooo-simulated drought stress. ThecodingsequenceofCnNAC83is786bpinlength，encoding261aminoacids，withaconservedNAMdomainatits N-terminus，anditssubcellularlocalizationispredictedtobethenucleus.Physicochemical propertyanalysisshowthathe isoelectricpointofCnNAC83is9.14，andagrandaverageofhydropathicity（GRAVY）score-0.725，indicatingitisahdro philicprotein.Phylogeneticanalysisshow that CnNAC83 ismostcloselyrelated toAaNAC83fromArtemisiaannua，while moredistantlyrelatedtoPgNAC83fromPunicagranatumandAtNAC83fromArabidopsis thaliana.RT-qPCRanalysis show thatdrought stresscan induceCnNAC83 expression.Theseresults indicate that CnNAC83 playsapositiveregulatoryrole in droughtresistanceinChrysanthemumnankingense，whichprovidesabasisforthestudyof thebiological functionof CnNAC83.

【KeyWords]Chrysanthemumnankingense;genecloning;NACtranscriptionfactor;droughtstress; expresionanalysis

[中圖分類(lèi)號(hào)］Q781 [文獻(xiàn)標(biāo)識(shí)碼]A [文章編號(hào)]1674-3229（2025）02-0107-08

0 引言

在植物的生命周期中，保持基因按照特定時(shí)空順序進(jìn)行表達(dá)對(duì)于維持其發(fā)育進(jìn)程至關(guān)重要，而在這一過(guò)程中轉(zhuǎn)錄因子扮演著不可或缺的基礎(chǔ)角色。（剩余9991字）