石河子市部分流浪犬源腸球菌耐藥性及耐藥基因檢測

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Abstract：To investigate the drug resistanceandresistance genecarriage status ofEnterococcus derived from straydogs in Shihezi CityXinjiang，thisstudycollects210 samples from thefecesand surrounding environment of straydogs. Enterococi wereisolated，purifiedandidentified byconventional bacterialisolationandidentificationmethods.Thedrug resistanceoftheisolates to14antibioticsisdeterminedthroughKirby-Bauer diskdifusionmethod，andthecariageof relateddrug resistance genes is detectedbyPCRtechnology.Atotalof163enterococi areisolated inthisstudyof which 119are from fecesand44were fromtheenvironment.These strains include23Enterocous faecalis，129 Enterococcus faecium，and11other Enterococcs.Theresultsof drugsensitivitytestshowthat Enterococcus from straydogs have high resistance tosulfamethoxazole，clindamycin，rifampicin，tiamulinand gentamicin，and theresistant strainsaccountfor 100% 78.53% 67.48% 63.19% and 53.37% ，respectively. In contrast，the resistance rates to penicillin，ampicillin and florfenicol are lower （2.45%-6.75%） .It is worth noting that no strains resistant to vancomycin，tigecycline and linezolid are detected. Theresults of multi-drug resistance indicate that they are mainly distributed in 4-6 resistance，accounting for 69.32% （113/ 163），of which 6 resistance accounted for the highest proportion （25.15%） ，while1 resistance account for the lowest proportion （1.23%） .In addition，the drug resistance of Enterococcus in the environment is slightly more serious than that in feces，andthedrugresistanceofEnterococcus faecium fromstraydogsisalsomoreseriousthan thatofEnterococcus faecalis.The results of drug resistance gene detection show that erm（B），aac（6'）/aph （2′′） ，aph （3′） -II，and tetMresistance genesaredetected，andoxazolidinonedrug-relatedresistancegnes （optrA，cf，oxtA），ancomycinresistancegene （van） andotherdrug resistance genes arenotdetected.Insummary，straydog sheltersare likelytobecomeapotentialreservoirof multidrug-resistantbacteriaandthereisariskofepidemicrelateddrugresistancegenes.Itissuggestedthatinthetreatment oflocalpetdogsandstraydogs，rationaldrug useshouldbecarriedoutaccording totheresultsofbacterialresistane，and themonitoringofbacterialresistanceinstraydogs shouldbestrengthened toreducetheriskof drug-resistant bacteria transmission.

Keywords：Stray dog;Enterococcus;Enterococcus faecalis;Enterococcus faecium; drug resistance

抗微生物藥物耐藥性（AMR）是全球性公共衛(wèi)生挑戰(zhàn)之一，嚴(yán)重威脅人類和動物健康[1]。（剩余17587字）