光譜流式細胞儀在生物材料檢測中的應用研究
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中圖分類號：Q631 文獻標志碼：A DOI：10.3969/j.issn.1007-7146.2025.03.004
Abstract：Asadetection instrument developed rapidlyinrecent years，the spectral flowcytometer primarilycomplemented traditionalflowcytometry.Thisarticledemonstratedtheperformanceof threespectralflowcytometers inthedetectionof fluorescent materials，demonstratingtheirpotentialapplications inthefieldofbiological materials.Studies showedthatthe spectral flowcytometercouldnotonlyexplorefluorescencefromnon-primarybandsofknown fluorescent dyes，but also decompose overlapping spectrabetween fluorescentmaterials，andreagentkitdetectionchannels，esulting inmoreaccurate analyticalresultsdditioallyituldtecttesctralsiftsoffuorestomaterialsiinell.Ialltstral flowcytometercouldrecordthespectralcharacteristicsoffluorescentmaterialsenteringcels，analyethespectralbehaviorof fluorophores within cells，and spectrallyresolve fluorescence signals that overlapped with detection channels，thus providing objective fluorescence data.These capabilities opened up new paths for the research offluorescent materials，providing theoreticalfoundationsaswellaspracticalreferencesforthewidespreaduseofspectralflowcyometryinbiomaterialsscience
Key words： spectral flow cytometry; biological materials; fluorescent materials; fluorescence spectrum shift;spectral separation
（ActaLaser BiologySinica，2025，34（3）：221-228）
近年來，流式細胞儀及流式細胞術(shù)發(fā)展迅猛，研究人員創(chuàng)新研究了多種類型的流式細胞儀，包括光譜流式細胞儀（spectrumflowcytometry）、質(zhì)譜流式細胞儀（masscytometry）、成像流式細胞儀（imagingcytometry）以及組織成像質(zhì)譜流式細胞儀（imagingmasscytometry）等。（剩余12814字）