山楂提取物調(diào)控高脂小鼠T細胞免疫及脂代謝的作用機制研究

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AbstractObjective：ToxplorethemechanismofhawthoextractregulatingTcelimmunityandiidmetabolisminhyperideic mice.Methods：Sixty healthymale6-weekoldspecicpathogenic（SPF）gradeC57BL/6Jmicewereadaptivelyraisedforoneweekand thenrandomly divided into the normal control group，high-fat diet（HFD） group，HFD + high-dose hawthorn extract group，HFD+mediumdosehawthomextractgroup，HFDlow-dosehawthomextractgroupandHFDatorvastatingroup.Thenormalcontrolgroupwasgiven ordinary feed andraised normaly for8weeks，while the mice in the HFDgroup were fed with high-fatfeed powered by 60% fat for 8 weks.MiceintheHFDhigh-dosehawthomextractgroup，HDmedium-dosehawthomextractgroup，ndHFDlow-dosehawthom extract group were gavaged with 5.2，2.6，and 1.3g/kg hawthorn extract，respectively after 4weeks of high-fat rearing，once a day.Mice in theatorvastatin groupwere given oraladministration of atorvastatinat a dose of 10mg/kg bygavageafter4weeksof high-fat feeding， onceaday.Thecontrolgroupandthe HFDgroupweregavagedwiththesamevolumeof normalsalineatthesamevolume.The intervention period was 4weeks.The mRNA and protein expression levels of CD36 and peroxidase proliferation-activated receptor γ （PPARγ） weredetected by WestemBlotand reverse transcription polymerasechain reaction（qRT-PCR）.Flowcytometry was used to detecthepropiosa）el）neli mice.Results：Compared with the normal group，the body weight of mice in the HFD group increased P<0.01 ）;theexpression levels of CD36 ， PPARγ mRNA，proteinincreased P<0.01 ）;the proportionof Th17 cels in the spleen increased，and the percentages of Treg and Tfhcells increased（ P<0.01 ）.Compared with the mice in the HFD group，the expression levelsof CD36 PPARγ mRNA，and protein inthe model groupmice intervenedwith hawthornextractand atorvastatindecreased P<0.01 ），thepercentagesofTregandTfhcellsincreased （P<0.01） ，andthepercentageofTh17celsdecreased P<0.01 ）.Theeffect of the HFD+high-dose hawthorn extract group and the atorvastatin group were significant（ P<0.05 or P<0.01 ）.Conclusion：Hawthorn extract can improve the imbalance of Treg，Th17 and Tfh cellsand the expression of CD36 PPARγ receptors in mice with dyslipidemiamodels.Lipid regulation by hawthorn extract may be related to T-cell immunity and lipid metabolism differentiation.

Keywordsdyslipidemia;Tcellimmunity; hawthorn extract; peroxisome proliferator-activated receptor γ; experimental study

心血管疾病（cardiovasculardisease，CVD）仍是全球首位死亡原因，據(jù)世界衛(wèi)生組織統(tǒng)計，約13的病人死于CVD，是當(dāng)今嚴重危害人類健康的疾病之-[1]。（剩余13942字）