石斛屬燈籠組物種葉綠體基因組特征及系統(tǒng)發(fā)育分析

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中圖分類號：Q943.2 文獻標識碼：A 文章編號：1007-0435（2025）05-1372-15

Abstract：To analyze the chloroplast（CP）genome structure and phylogenetic relationships of section Densiflora i the genus of Dendrobium，this studyused high-throughput sequencing technology for the first time to sequence the CP genomes of four species in the section，including D . farmer，D. palpebrae，D. sulcatum，and D.thyrsiflorum. The genomes of section Densiflora were assembled，annotated，mapped and analyzed by bioinformatics software.Phylogenetic trees of 42 species of Dendrobium，including ten species of section Densiflora，were constructed based on cp genomes. The complete cp genomes of section Densiflora were 151 717- 160 123 bp in size with 37.1%-37.6% GC content. Additionally，46-63 simple sequence repeats（SSRs） and 48-49 interspersed nuclear elements （INEs）were identified in section Densiflora. Codon usage in proteincoding genes is biased toward A and U（or T）.There were significant differences inthe coding regions of six genes， including trnKUUU ， at?F ，rpoC， trnLUAA ，clpP and among section Densiflora. Phylogenetic analyses revealed that ten species of section Densiflora were clustered into four diferent clades，indicating that this section defined by traditional morphology is non-monophyletic. Key words：Dendrobium section Densiflora;Chloroplast genome;Genomic comparison;Phylogenetic analysis

石斛屬（Dendrobium）隸屬蘭科（Orchidaceae）石斛亞族（Dendrobiinae），與石豆蘭屬（Bulbophyl-lum）構(gòu)成姐妹類群[1]。（剩余20130字）