山茶WRKY基因家族鑒定及其在花色形成中的表達(dá)分析

打開文本圖片集

中圖分類號(hào)：S794.9 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1673-923X（2025）07-0188-09

Identification and expression analysis of WRKY gene family in flower color formation in Camellia japonica

SONGZhixin12，F(xiàn)ANMenglong1，JIANGHong1，ZHANGYing1，LIXinlei1 （1.ResearchInstituteofbtropicalForestryineseAcademyofForestry，Hangzhou340o，Zhejang，ina 2.Nanjing ForestryUniversity，Nanjing21oo37，Jiangsu，China）

Abstract：【Objective】The WRKYgene familyplays animportantrolein theregulation of plant growthand development.Camelia japonicaisanimportantoamentalplantwithichfowercolors.ItisofgreatsignificancetoidentifyandanalyzetheWRKYgene familymembers inCamelia japonica petals withdiferentcolors.【Method】Basedonthe whole genome dataofCameliajaponica， theWRKYgenefamilywasidentifedandanalyzedbybioinformaticsmethods.Basedonthetranscriptomedatarelatedtocamellia flowercolor，teexpressionpateofCjWKYsindifentcoloedamellapealsasaalyed【Result】esultsindcatedtat 94 CjWRKYgenes were identified in the whole genomeand named CjWRKY1-CjWRKY94.The phylogenetic tree dividedthe strains intothregos（I，I）adgopIIwafurthvieditfiveubous（a，I-b-c，I-I-e）.ob contained twoWRKYdomains，whilegroupI andIImemberscontainedonlyone WRKYdomai.Atotalof10conservedmotifsand 5conserveddomainswereidentifed.TheCjWRKYsmembersinthesamesubgrouphadsimilarconservedmotifs，conserveddomains and genestructures.The CjWRKYs promoterregioncontained 38 elements forlightresponse，stressresponseandhormonerespose， includingMYBbindingsiteforregulatingfavonoidbiosynthesis.94CjWRKYswereunevenlydisrbutedon15chromosomes，and there were13 pairsoftandemduplication events.Collnearityanalysisshowed thatthere were72duplication events in94CjWRKYs genes，and the Ka/Ks valueof the duplication genes was less than1.There were 116 colinear gene pairs with Arabidopsis WRKY gene family.Amongthem，32CjWRKYs wereup-regulatedand6CjWRKYsweredown-regulated withthedeepeningofflowercolorof Cameliajaponica，whichsugestedthat CjWRKYs plaedanimportantroleinteregulationofanthocyanisyntesis.Itisspeclated thatCjWRKY48，CjWRKY53andCjWRKY6 positivelyregulateanthocyaninsynthesisbyinteracting withMYBtranscriptionfactors， therebyaffectingflowercolorofCameliajaponica.【Conclusion】94CjWRKYsgeneswereidentifedinthewholeCamellajaponica genome，andmostCjWRKYsareinvolvedinanthocyaninsynthesisandplayanimportantroleincolorformationofCameliajaponica.

Keywords：Camellia japonica;WRKYgene family;flowercolor;expressionanalysis

WRKY基因家族是高等植物中最大的轉(zhuǎn)錄因子家族之一，參與植物中大部分生理代謝過(guò)程，包括調(diào)控生長(zhǎng)發(fā)育、參與脅迫響應(yīng)以及次生代謝物生物合成等[]。（剩余17502字）