杜仲葉片不同發(fā)育時(shí)期轉(zhuǎn)錄組及綠原酸相關(guān)基因分析
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中圖分類號(hào)：S792.99 文獻(xiàn)標(biāo)志碼：A 文章編號(hào)：1673-923X（2025）07-0174-14
Analysisof transcriptomeand chlorogenicacid related genesin Eucommia ulmoides leaves at different developmental stages
WANGSiran1，LIHui，Aricha’，JINMulan2，QINGJun1，BAIYu’e1 （1.CollegeofForestryInnerMongoliaAgriculturalUnversity，Hohhot Ooo19，Inner Mongolia，China; 2.UlanqabForestryand GrassandMonitoring andPlanning Station，UlanQab O120o，Inner Mongolia，China）
Abstract：【Objective】Chlorogenicacid isanindex componentforthequalityevaluationofEucommiaulmoides leaves，whichhas variousmeiciallusschtbactealdtamtorytiodanttacrdtptitieatio andexcaationofmetabolicpathwaysandgnesinvolvedinregulatingchlorogenicacidbiosynthesis inEucommiaulmoidesleaves willprovidefurtherevidenceforanalysisofthemolecularmchanismofchlorogenicacidformationinEucommiaulmoidesleaves. 【Method】Eucommialmoidesleavesrecectedfromotou，Iergoliautonomousgion，inpril，Julydctober 2023at diferetdevelopmenalperods，andwereclaifedaccordingtotecharacterationas：Aprilfreshlyunfoledyoungleaves; Julymatureleaves，withlighteathery;andctoberfullymatureaves，withthdarkestcoloroftelavesandompleteleathrye chlorogenicacidcontentofEucommiaulmoidesleavesatdiferentperiodswasdeterminedbyig-performanceliquidchromatography （HPLC），andthediferetiallexpressedgenesereidentifdbyrasiptomesequencing.【Result】Teloogniccidctet ofEucommiaulmoidesleavesrangesfromApriltoJulyandthentoOctober，showsanincreasingandthendecreasing trend withleaf development.Transcriptome sequencing yielded 58.06 Gbof clean data，enablingthe identification of11 689 genes.The numberof down-regulatedexpressdgnesisigherthanteumberofupregatedexpresedgenesinteompasonofsTTsand
T2vsT3hreegroups.KEGGenrichmentanalysisandGOenrichmentanalysis，bothofwhichdisplaychlorogenicacidsynthsis-related enrichments.FurtemoreKmeanstredexpreionividsteensinto0modles，inwhichtrendsofgeneexpressinlass4， Class8adClassareconsstentwithtrendsoforogenicacidotetadtwozsandegensareidentifdfroClass4 Class8andClass10tatarerelatedtoclorogenicacidbiosythsis.Tresultsofal-tiefuoreseequanitativePCR（-CR） are inaccordance withRNA-seqoutcomes.【Conclusion】ThechlorogenicacidcontentintheEucommiaulmoides leavesshowsa trend of increasing and then decreasing with the development of the leaves.
Keywords：Eucommiulmodesorogeniccid;trsiptoe;difrentiallyexpreedneDEGs）;iofoatiais
杜仲Eucommiaulmoides落葉喬木，為杜仲科杜仲屬植物，是我國(guó)名貴藥材之一，亦是藥食同源植物。（剩余16746字）