短梗木霉與芽孢桿菌固體共培養(yǎng)物WP劑型研制

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中圖分類號：TQ450.6 文獻標志碼：A文章編號：1673-9868（2025）05-0090-13

Preparation of Wettable Powder for Solid Co-Culture of Trichoderma breve LNoo6 and Bacillus velezensis ZJ

WU Yiping1， WANG Jingwen1， ZHANG Yaxuan1， LIU Tong1，2

1.Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests of Ministry of Education/School of Tropical Agriculture and Forestry，Hainan University，Danzhou Hainan 571737，China; 2.School of Breeding and Multiplication，Hainan University，Sanya Hainan 572020，China

ibstract： Trichoderma breue LNoo6，which has a growth-promoting effect and disease prevention effect on plants，and Bacillus velezensis ZJ were used as functional strains to prepare a wettable powder （WP） by solid co-culture，grinding，and mixing with carriers and dispersants. The effect of its application on rice sheath blight was measured. The results showed that the number of spores of Trichoderma in the co-culture products was 7.51×109CFU/g ， and the number of bacterium was 9.22×109 CFU/g，and the optimal formula of wettable powder was as follows： 20% product of co-culture， 66.6% hydrated silica， 5% Tween-20， 8% sodium carboxymethylcellulose and 0.4% lecithin. The wetting time of the formula was 41.72s ， the suspension rate was 87.79% ，the pH value was 7.11，the pass rate of 20O mesh sieve was 95.77% ，the number of live spores of Trichoderma was 1.45×109CFU/g ， and the number of live bacterium was 1.78×109 CFU/g. The foaming property is 14mL ，which meets the requirements of the national standard. There was no significant difference in control effect on rice sheath blight between WP （65.12% ） and control agent Jinggangmycin （72.09% ）. The germination test showed that the plant height and root length of WP treated rice were increased by 18.67% and 10.47% compared with the control， respectively. （20 T . breue LNoo6-B. velezensis ZJ WP provides a new way for green and sustainable prevention and control of rice sheath blight.

Key words： Trichoderma breoe ; Bacillus velezensis ； compatible strains; co-culture； wetble powder

水稻紋枯病是由立枯絲核菌（Rhizoctonia soani）引起的土傳真菌病害，在秧苗期至穗期均可發(fā)生，可造成嚴重減產(chǎn)。（剩余13701字）