津強11號小麥醇溶蛋白缺失突變體篩選及利用

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ScreeningandUtilizationofWheatGliadinDeletionMutantinJinqiang11

HANG Xinyang1， QIN Shaoning'， WANG Zhishangl， ZHANG Jingdan'， CHEN Huafeng23， ZHANG Jianingl （1.CollegeofAgronomyandiotechnology，HebeiNoalUniversityofSienceandTechnologHbeiKeyLaboratoryofCotr Biology，Qinhuangdao，Hbei6O，ina;2.anjinKeyaboratoryofCropGeneticsandBreeding，anjinropesearchstitute，Tianjincadmyofgiculturaliencs，njinO84，hina;3.anjinZogtianDadiTchologompanyLied jin 300384，China）

Abstract：Inordertocreateandsrennewresourcesof high-qualitywheat，theresearchersusedthe mutagenicprogenyofJinqiang 11 through EMS to screen the gliadin deletion mutants.The results revealed that a total of four γ2 -gliadin deletion mutants were obtained，namedJQ11-8，JQ11-62，JQ11-64，andJQ11-65，espetivelyGrainpenotpeaalsisshowedtattegrainlgthad grain width of the four γ2 -gliadin deletion mutants were not significantlydifferent from those of Jinqiang 11.The analysis of quality traits showed that the protein content and gluten protein content of the four γ2 -gliadin deletion mutants were significantly higher than thoseofJinqiang11，andthstarchcontentwassignificantlylowerthanthatofJinqiang11.OnlythestabilitytimeofJQ11-65and the SDSsedimentationvoumeofJQ11-58showednosignificant diferencecomparedwith thoseofJinqiang11.Inconclusion，the discovery of four γ2 -gliadin deletion mutants provides a new resource for creating high -quality wheat.

KeyWords：wheat; gluten; gliadin; processing quality; mutant

小麥（TriticumaestivumL.）是我國重要的糧食作物，為人類日常飲食提供 20% 的熱量和蛋白質(zhì)1。（剩余13348字）