不同來源廣藿香葉綠體基因組研究
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中圖分類號：Q943.2 文獻標(biāo)識碼：A 文章編號：1000-3142（2025）04-0716-14
Chloroplast genome analysis Pogostemon cablin from different origins
XING Bingnan，LIANG Yingying，WU Wenru*，LU Yaru， ZOU Heyuan，PENG Xiaoqi （SchoolPharmaceutical Sciences，Guangzhou 51Ooo6，China
Abstract：Pogostemoncablinpossssssignificant medical and industrialvalues，as itcanbe usedfor medicinal purposes as well as for essential oil extraction. However，the yield and quality P . cablin can vary depending on the ecological environments andartificial cultivation measures employed in diferent production regionsandorigins.Inorder
to study the structural characteristics and compare the diferences the chloroplast genome P .cablindifferent origins，this study used the DNBSeq sequencing platform to sequence the whole genome P . cablin，used GetOrganelle to assemble the complete chloroplast genome，annotated the chloroplast genome on the CPGAVAS2 website，and analyzed the basic structural characteristics，IR/SC boundary comparison，genome comparison and collinearityanalysis， simplerepeat sequences and interspersed repeat sequences，diversityanalysis and relative usage analysis synonymous codons.The results were as follows：（1）The fullength the chloroplast genomes 2O different origins P .cablin was 152 461-152 510 bp，and 132 genes were annotated，including 87 CDSs，37tRNA genes and 8 rRNA genes.（2） The mVISTA comparison found that atpF，atpF-atpH，rps16-trnQ-UUG，rpoB-trmC-GCA，accD，psaI-ycf4，petApsbJ，rpl16，andrps15-ycfl were hypervariableregions.（3）Thesites withnucleicaciddiversitygreaterthanO.002 were located in the trnM-CAU-atpB interval，ycf4，rpl32，and rpl32-trmL-UAC interval.（4）Atotal 64codons encoding 20aminoacidsweredetected，andthere were 33 highly preferred codons，among which codons ending inA/Uaccounted forthemajority.（5）And74-76 SSRs，15-18 palindrome repeat sequences，and12-17forward repeat sequences were detected.（6）Genetic distance analysisand phylogeneticanalysis found that only GSY_MLXY hada distant genetic relationshipwithothercultivatedtypes.Inthisstudy，the genomestructure informationanddiferentsiteschloroplasts from 20 different sources P . cablin are obtained，which provides basic data for the development molecular markers and the screening superior germplasm.
Key words： Pogostemon cablin，chloroplast genome，repetitive sequence，diversity，relative synonymous codon usage
廣藿香（Pogostemoncablin）為唇形科刺蕊草屬植物，以干燥地上部分入藥，其功效為芳香化濁、開胃止嘔、發(fā)表解暑（國家藥典委員會，2020），廣泛應(yīng)用在醫(yī)藥、食品和化妝品加工業(yè)等方面，具有較高的經(jīng)濟價值，亦是新型冠狀病毒肺炎防疫中的重要中藥（李浩等，2021）。（剩余15919字）