核受體NURR1在前列腺癌細(xì)胞中的表達(dá)及其對(duì)環(huán)狀RNA表達(dá)譜的影響

王宏亮 梁輝 鄧瓊 張穎 王鑄

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Expression of nuclear receptor NURR1 in prostate cancer and its effects on the circRNA profile

WANG Hongliang，LIANG Hui，DENG Qiong，ZHANG Ying，WANG Zhu

（Department of Urology，Shenzhen Longhua Hospital Affiliated to Southern Medical University，Shenzhen 510080，China）

ABSTRACT：

ObjectiveTo investigate the expression profile of circRNA in nuclear receptor NURR1 overexpressed prostate cancer （PCa） cells，so as to provide reference for revealing the mechanism of PCa progression.MethodsThe expression of NURR1 in PCa was analyzed with UALCAN and TNMplot. The distinct circRNAs in NURR1 overexpressed PCa cells were screened with RNA，sequencing. The functions and signal pathways of differentially expressed circRNA molecules were analyzed with GO and KEGG. ResultsThe circ_0000915 was significantly downregulated in DU145，LNCaP and PC3 cells. In NURR1 overexpressed DU145 cells，circ_0005991 was up，regulated，while circ_0001460 and circ_0001315 were down，regulated. In NURR1 overexpressed LNCaP cells，circ_0040729 and circ_0000722 were significantly up，regulated. In NURR1 overexpressed PC3 cells，circ_0001577，circ_0000854 and circ_0018168 were up，regulated，while circ_013035，circ_0003028，circ_0082096 and circ_0005320 were down，regulated. KEGG analysis revealed that the differentially expressed circRNAs were significantly associated with dorsal/ventral neural tube patterns，protein folding chaperones，disordered domain specific binding，positive regulation of BMP signaling pathways，and neural tube patterning functions. ConclusionCircRNAs play an important role in NURR1 mediated PCa progression，but there are certain differences among different prostate cancer cell types. The regulatory mechanism between NURR1 and circ_0000915 in the progression of PCa needs further investigation.

KEY WORDS：nuclear receptor；NURR1；prostate cancer；circRNA；invasion and metastasis；DU145；LNCaP；PC3

摘要：目的探討核受體NURR1過(guò)表達(dá)對(duì)前列腺癌（PCa）細(xì)胞中環(huán)狀RNA（circRNA）表達(dá)譜的影響，為揭示NURR1相關(guān)circRNA分子在PCa進(jìn)展過(guò)程中的作用提供參考。（剩余10517字）