熒光壽命成像技術(shù)及其在生物醫(yī)學(xué)領(lǐng)域的應(yīng)用

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關(guān)鍵詞：熒光壽命成像技術(shù)；頻域法；時(shí)域法；生物醫(yī)學(xué)中圖分類號(hào)：TN247；0657.3 文獻(xiàn)標(biāo)識(shí)碼：ADOI：10.37188/CJL.20240240 CSTR：32170.14.CJL.20240240

Fluorescence Lifetime Imaging Technology and ItsApplicationsin Biomedical Science

YANWanlu1，SUWeitaol*，CHENDaqin2 （1.SchoolofScience，HangzhouDianzi University，Hangzhou31oooo，China; 2.School ofPhysicsand Energy，F(xiàn)ujianNormal University，F(xiàn)uzhou 35oooo，China） *CorrespondingAuthor，E-mail：suweitao@hdu.edu.cn

Abstract：Fluorescence lifetime imaging is atechnique widelyused in various fields.Ithas high sensitivity，high timeresolutionandspatialresolution.Moreover，italsocan beused toquantitatively study thesamplewithoutthe influence of excited laser and environmental factors.Because of the above advantages，fluorescence lifetime imaging hadbeenwidelyused inbiomedicalfield.Fluorescencelifetimeimaging techniquecanbedivided into frequencydomain and time domain routines.Frequency domain routinecanbe divided into heterodyne method and homodyne methodaccording tothedifference frequency characteristics.There arethree major methods torealize time-domain routine，i.e.gating method，time-correlated single photon counting method（TCSPC）and scanning camera measurement method.This work introduces the basic principles of aforementioned fluorescence lifetime imaging methods. Wethenreview the recent application offluorescence lifetime imaging in biological sciencesandthe clinic study of tumorcancercell，and further give itsperspectives.

Keywords：fluorescencelifetimeimaging technology；frequencydomainmethod;timedomainmethod;biomedicalscience

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