河北楊PhMYB14基因的克隆及生物信息學(xué)分析

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Cloning and Bioinformatics Analysis of PhMYB14 Gene in Populus hopeiensis

Pang Linlin，Zhang Qi，Dai Jinling，Bai Yu'e （College of Forestry， Inner Mongolia Agricultural University，Hohhot O1Oo1O，China）

AbstractMYB transcription factors play important roles in plant growth and development in ways of interactions，signal transduction，and gene expresson regulation.In this study，using the seedlings of Populus hopeiensis as materials，PhMYB14 gene was cloned，its bioinformatic analysis was performed，and its tissuespecific expression in P . hopeiensis was analyzed. The results showed that the length of coding sequence （CDS） of PhMYB14 gene was 723bp ，encoding 240 amino acid residues. The PhMYB14 protein had the molecular weight of 27 512.87 Da and the theoretical isoelectric point of 5.17，and was an unstable hydrophilic protein with no signal peptide and transmembrane structure. Homologous sequence alignment and protein phylogenetic tree analysis showed that PhMYBl4 was the most closely related to Populus trichocarpa and Populus alba，both of which also belonged to the genus Populus，followed by Salix sinopurpurea and Salix suchowensis. Prediction of subcellular localization suggested that PhMYB14 was mainly locatted in chloroplasts.The promoter region of PhMYB14 contained various cis-acting elements including photoresponsive elements and methyl jasmonate response elements.Real-time fluorescence quantitative analysis （qRT-PCR） showed that PhMYB14 was expressed in the roots，stems and leaves of poplar seedlings with the highest expression level in leaves. The results of this study could provide a reference for further study of the functional mechanism of PhMYB14 in the resistance to diseases and insect pests of P . hopeiensis.

KeywordsPopulus hopeiensis ； PhMYB14; Gene Cloning； Bioinformatics; Expression analysis

轉(zhuǎn)錄因子（transcriptionfactor，TF），又稱為反式作用因子，可以與基因啟動子區(qū)域的順式作用元件相互作用，對轉(zhuǎn)錄過程起到抑制或激活作用，進而調(diào)控植物的生長發(fā)育。（剩余11338字）