甜瓜掌狀裂葉p基因?qū)A藏期果實(shí)后熟生理的調(diào)控作用

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Regulatory effect of the palmate-lobed leaf gene pll on the post-harvest physiology of melon fruits during storage

ZHANG Qiang1， YONG Liangmin1， DAI Wenting2， SHI Yunjiao1

（1.Hinanlsfse

al Sciences，Haikou 571100，China）

Abstract： The melon palmate-lobed leaf gene pll belongs to AINTEGUMENTA（ANT） transcription factor gene， which playsan importantrole inregulating plant growthand fruit development.During theresearch，the gene silencing of pll was detected byvirus-induced gene silencing（VIGS）technique.Thechanges of lipoxygenase activity，ethylene release and respiratory metabolism in fruits under normal and silent expression of pll gene were compared，and the effects of pl （204號(hào) gene silencing inhibitiononthe expression of ethylene synthesisand sugar metabolism enzyme-encoding genes and related enzyme activities in fruitswere studied.The results showed that the low expression of pll gene in fruits could reduce the activityof lipoxygenase，ethylene release and respiration rate.Moreover，the pll gene had a positive regulation efect on ethylene synthesis and carbohydrate metabolism gene expression in fruits，and when the expression of pll gene was suppressed by silencing，the expresion of genes related to ethylene synthesisand glucose metabolism in fruits was also dow-regulated， thus reducing the activities of ethylene synthesis and glucose metabolism enzymes.It can be seen that the pll gene is in

收稿日期：2024-10-29

volvedintheregulationofpost-harvestphysiology，andthe down-regulation of pll gene expression has a delayed and inhibitory effect on the post-harvest physiological metabolismof melon fruits.

Key words：muskmelon； pll gene； gene silen-cing；enzymeactivity；post-harvest physiology

甜瓜（CucumismeloL.）主要產(chǎn)于中國(guó)西部地區(qū)，其農(nóng)藝性狀優(yōu)良，果型勻稱，口感與風(fēng)味俱佳，深受消費(fèi)者喜愛(ài)。（剩余11405字）