22份狼尾草屬材料和1份蘆竹屬材料染色體數(shù)目及遺傳多樣性分析

打開文本圖片集

Abstract： Pennisetum and Arundo are important species of Juncao，and some of them have the problems in name confusion and inaccurate chromosome number. In this study，chromosome sectioning technology，SRAP and SSR molecular markers were used to identify the number of chromosomes and analyze the genetic diversity of 22 materials of Penmisetum and 1 species of Arundo （Arundo donax ‘Lyuzhou No.1'）. The results of chro mosome counting showed that the chromosome number of Penmisetum was 28，allof them were tetraploid， while the chromosome number of Arundo donax‘Lyuzhou No. was 110. A total of 296 bands were amplified by twelve pairs of SRAP primers and six pairs of SSR primers，including 277 polymorphic bands. The polymorphic rate was 9 5 . 5 % . The genetic distance ranged from O.O6l to O.9O5. The cluster analysis showed that 23 materials were classified into 7 groups with a genetic similarity coeffcient of O.77. The first category included 13 materials，including（P. glaucum × P . purpureum） × P purpureum‘Guimu No.1’，P. purpureum × P tyhoideum‘RY No. ，P. glaucum × P ：purpureum- FD and so on. P. purpureum‘Mott’，P.

purpureum‘Purple’ and P ：purpureum- JC-ZJ were clustered into the second，third and sixth categories， respectively. P ：purpureum‘Guiminyin’and P ：purpureum from Xichang were clustered into the fourth category. The remaining four P . purpureum were clustered into fifth category. Arundo donax‘Lyuzhou No.1' was clustered into the seventh category at the bottom，which had the farthest relationship with other Penisetum materials.Through chromosome number identification and molecular markers，the 22 materials of Penmisetum andl material of Arundo were accurately identified and the genetic relationship between them was analyzed， which provided some reference for standardizing the names of market materials and variety breeding. Key words： Pennisetum；Arundo； Chromosome number；SRAP ；SSR； Genetic diversity

菌草是指所含營養(yǎng)適用食用菌、藥用菌等微生物生長需要，且具有綜合開發(fā)利用價值的草本植物，包括蘆葦屬（Phragmites）、狼尾草屬（Pennis-etum）蘆竹屬（Arundo）芒屬（Miscanthus）等20多個屬，不僅可作為食（藥）用菌培養(yǎng)基，還在生態(tài)環(huán)境建設(shè)、畜牧業(yè)發(fā)展以及能源開發(fā)利用等方面發(fā)揮著重要作用1。（剩余12560字）